Methods in Renal Toxicology

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CRC Press, Mar 14, 1996 - Medical - 448 pages
The only up-to-date compilation of renal methods available, this book is the definitive resource for any renal researcher eager to stay ahead. Methods in Renal Toxicology presents a vast array of methods for the study of renal cellular and tubular structure, function, and biochemistry under physiological, toxicological, and pathological conditions. It provides detailed descriptions in easy-to-understand language of methods designed to enhance your research efforts.
Methods in Renal Toxicology puts you on the cutting edge with valuable chapters detailing molecular methods and transgene and gene targeting - the most recent approaches to the study of renal toxicology. Overall, the book's topics range from non-invasive assessments of renal function in the whole animal and clinical settings to cellular and molecular approaches. Specifically, the book delves into measurements of clearance and urinary markers, histopathology, and methods to assess renal carcinogenesis, mutagenesis, oxidative stress, mitochondria injury, cellular repair, and drug metabolism and transport. A variety of in vitro methods are also described, including the isolated perfused kidney, micropuncture, microperfusion, microdissection, renal slices, isolated perfused tubules, suspensions of tubules and isolated cells, and primary cell cultures and cell lines.
Methods in Renal Toxicology is a must-have resource for all renal investigators. Nowhere else can you find concise descriptions of traditional and up-to-the-minute renal toxicology methods in such a practical, well-written single-volume guide.
 

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Contents

Clearance Methods in Renal Toxicology
1
Urinary Markers
19
Histopathologic Methods in Renal Toxicology
35
The Isolated Perfused Kidney
59
Micropuncture and Microperfusion Techniques
79
Microdissection and Microanalysis of Specific Nephron Segments from Mammalian Kidney
97
Preparation and Use of PrecisionCut Renal Cortical Slices in Renal Toxicology
109
In Vitro Perfusion of Isolated Nephron Segments A Method for Renal Toxicology
123
Immortalized Cell Lines of Renal Cells
217
Methods in Renal Carcinogenesis and Mutagenesis
237
Measurement of Reactive Oxygen Species Oxidative Damage and Antioxidant Systems in the Kidney
255
Mechanisms of Repair After Renal Injury
283
Mitochondrial Toxicity in Renal Injury
299
Biotransformation and Membrane Transport in Nephrotoxicity
331
Methods to Evaluate Renal Cytochrome P450 as a Bioactivation System for Nephrotoxicants
353
Molecular Methods in Renal Toxicology
377

Suspensions of Rabbit Renal Proximal Tubules
147
Characterization and Use of Rabbit Renal Proximal Tubular Cells in Primary Culture for Toxicology Research
163
Use of Freshly Isolated and Primary Cultures of Proximal Tubular and Distal Tubular Cells from Rat Kidney
189
Transgenic Animals
413
Index
427
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