G Protein-Coupled ReceptorsGabriel Berstein Covering recently developed methods in membrane-bound receptors, this book emphasizes receptor structure and function, knowledge of which is essential to the study of signal transduction. G Protein-Coupled Receptors has culled contributors from domestic and international sources, providing a broad base of knowledge. Some topics covered are the r |
Contents
| 1 | |
Ligand Screening of G ProteinCoupled Receptors in Yeast | 49 |
Ligand Screening Using Melanophore Cells Frog Skin to Combinatorial Chemistry | 71 |
A Screening Orphan Receptors for Native Ligands | 97 |
Reconstitution of ReceptorPromoted Signal Transduction Using Purified Receptor G Protein and Effector | 117 |
Mutagenesis Approaches for Studying ReceptorG Protein Interactions and Receptor Assembly | 155 |
G ProteinCoupled Receptor Kinases and Desensitization of Receptors | 185 |
Internalization and Downregulation of G ProteinCoupled Receptors | 211 |
Use of Electrophysiology to Monitor and Study Receptor Desensitization | 239 |
Expression in Escherichia coli and LargeScale Purification of a Rat Neurotensin Receptor | 265 |
LargeScale Expression of Receptors in Yeasts | 281 |
Electron Crystallography of Membrane Proteins | 323 |
XRay Crystallography of Membrane Proteins from Detergent Solution The Structures of a LigandGated Bacterial Membrane Receptor in Two Confor... | 355 |
XRay Crystallography of Membrane Proteins Concepts and Applications of Lipid Mesophases to ThreeDimensional Membrane Protein Crystallization | 365 |
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2D crystals activity adenylyl cyclase affinity agonist allosteric amino acids analysis antagonist antibody bacteriorhodopsin Biochem Biol buffer C-terminal centrifugation cerevisiae channel Chem CHO cells clones coli column concentration cubic phases culture desensitization detergent determined dilution dissociation EDTA electron electron crystallography eluted expression FhuA Figure filter fluorescent fractions functional fusion G protein G protein-coupled receptors gene GPCRs HEK 293 cells incubate inhibition inhibitor interactions intracellular ligand lipid M₂ mammalian measured medium melanophores membrane proteins method mg/ml molecular molecules muscarinic acetylcholine receptor muscarinic receptor mutant NaCl nonspecific binding orphan receptors pastoris peptide Pharmacol pheromone pipette plasmid plates pmol/mg preparation production Protocol purification radioligand radioligand binding Receptor Internalization receptor kinase recombinant reconstitution residues resuspend rhodopsin room temperature samples screening sequence signal transduction solubilized solution specific structure subunits supernatant surface transfected tubes unlabeled vectors vesicles Wash yeast yeast cells µg/ml