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LESSONS AND LABORATORY EXERCISES

IN BACTERIOLOGY.

SMITH.

LESSONS AND LABORATORY

EXERCISES

IN

BACTERIOLOGY

AN OUTLINE OF TECHNICAL METHODS INTRODUC-
TORY TO THE SYSTEMATIC STUDY AND

IDENTIFICATION OF BACTERIA,

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PRESS OF WM. F. FELL & CO.. 1220-24 SANSOM ST..

PHILADELPHIA.

COPYRIGHT, 1902, BY ALLEN J. SMITH.

G65 564 1902

PREFACE.

The following pages were originally arranged as a series of exercises to be carried out by the class in the University of Texas under the guidance of an assistant, the hours of work of the several classes so overlapping that it was impossible for the writer to give his personal attention to the class in this laboratory, for the work in which he was, however, responsible. The exercises were outlined daily upon the blackboard, and verbal instructions as to their purpose and the manner of procedure given, as required, to the class. The arrangement being found well adapted to systematic work, it has been continued, with changes from and some additions to the original scheme, for several years; and recently, at the request of some of his students and with the thought that perhaps others might find some such definite arrangement of work of use in teaching, the writer has written out these exercises for publication. It has seemed advantageous that in their published form there should accompany the practical exercises such explanatory matter as would adapt the book as a student's laboratory outline guide. It would be beyond the writer's intention to make the work a compendium of methods, and the instructions, although forming a considerable bulk of the volume, include only such as from his experience he has adopted as best suited in class work.

There are arranged blank pages upon which notes of the outcome of the various experiments and record of special instruction as to technique may be added; and as an appendix a blank form is printed, following which as a form at the close of the work should be recorded the data ascertained in connection with the more important forms of microorganisms which have been studied in the exercises.* It has been the writer's custom to have each student carry along with the regular class work during the last two or three weeks some simple independent task, as the bacterial analysis of a water-supply, of milk, soil, or air, in which an enumeration of the bacteria found in a definite quantity of the substance examined is required, together with the identification of one or more forms of the organisms encountered, and such study of their pathogenic influence as time permits. The records of such work also may well be made after the blank form in the appendix. *Copies of this form may be obtained from P. Blakiston's Son & Co., Philadelphia.

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