CONTENTS.

INTRODUCTION.

THE GERM THEORY

PART I.

GENERAL METHODS.

CHAPTER I.

APPARATUS, MATERIAL, AND REAGENTS EMPLOYED

IN A BACTERIOLOGICAL LABORATORY.

PAGE

I

23

a. Histological apparatus

23

b. Reagents and materials employed in the processes of hardening,

decalcifying, embedding, fixing, and cutting of tissues.

25

c. Reagents for examining and staining microscopical preparations

d. Reagents for mounting and preserving preparations

27

34

e. Drawing and photographic apparatus

34

f. Sterilisation apparatus

g. Apparatus and material for preparing and storing nutrient gelatine

and nutrient agar-agar

36

38

h. Apparatus for employment of nutrient jelly in test-tube and plate-

i. Apparatus for preparation of potato-cultivations

j. Apparatus for preparation of solidified sterile blood serum

k. Apparatus for storing, and for cultivations in, liquid media

m. Inoculating and dissecting instruments and apparatus in common use

#. General laboratory requisites

41

45

46

48

49

58

59

a Yugsun of nutrient gelatine and nutrient agar-agar

of employing nutrient jely in test-tile zas plate-culty

to jure and employment of sterilised potatoes, potato-paste,

pante, vegetables, fruit, and white of egg

are and employment of sterile blood serum

MYDIA:

Ꮡ

104

G» for the f sterilised bouillon, liquid blood serum, urine, milk,

gaze infusions, and artificial nourishing liquids

Adeka di storing and employing liquid media: Lister's flasks,

106

test-tubes, Sternberg's bulbs. Pasteur's apparatus,

bios; Drop-cultures; Warm stages.

109

CHAPTER V.

EXAMINATION OF AIR, SOIL, AND WATER

Air

Soil

Water.

CHAPTER VI.

EXPERIMENTS UPON THE LIVING ANIMAL

a. Inhalation of micro-organisms

b. Administration with food

c. Cutaneous and subcutaneous inoculation

d. Special operations

CHAPTER VII.

EXAMINATION OF ANIMALS EXPERIMENTED UPON,

AND THE METHODS OF ISOLATING MICRO-ORGAN-

ISMS FROM THE LIVING AND DEAD SUBJECT

a. Method of dissection and examination

b. Isolation of micro-organisms from the living subject

PART II.

GENERAL BIOLOGY OF BACTERIA.

CHAPTER VIII.

GENERAL MORPHOLOGY AND PHYSIOLOGY

CHAPTER IX.

PAGE

125

125

130

131

137

137

137

138

139

ANTISEPTICS AND DISINFECTANTS .

180

IMMUNITY

CHAPTER X.

192

F. Table showing the magnifying power of Zeiss' objectives

LIST OF ILLUSTRATIONS.

FIG.

WOOD ENGRAVINGS.

1. Koch's Steam-Steriliser

2. Hot-air Steriliser

3. Section of Hot-air Steriliser

4 Hot-water Filtering Apparatus, with Ring Burner 5. Wire Cage for Test-tubes

24 Siphon Bottle, with Flexible Tube, Glass Nozzle, and a Mohr's Pinchcock

25. Dessicator

26. Method of making a Folded Filter

27. Method of Inoculating a Test-tube, containing Sterile Nutrient Jelly 28. Method of Inoculating Test-tubes in the preparation of Plate-cultivations

29. Method of dividing Potatoes

30. Method of Forming a Simple Moist Chamber

31. Simple Warm Stage

32. Simple Warm Stage shown in Operation

33. Schäfer's Warm Stage.