Principles and Reactions of Protein Extraction, Purification, and CharacterizationPrinciples and Reactions of Protein Extraction, Purification, and Characterization provides the mechanisms and experimental procedures for classic to cutting-edge techniques used in protein extraction, purification, and characterization. The author presents the principles and reactions behind each procedure and uses tables to compare the different |
Contents
| 1 | |
Chapter 2 Estimation of Protein | 35 |
Chapter 3 Electrophoretic Analyses of Protein | 71 |
Chapter 4 Purification of Protein | 133 |
Structures Interactions and Production | 193 |
Other editions - View all
Principles and Reactions of Protein Extraction, Purification, and ... Hafiz Ahmed,Hafiz Ahmed PhD No preview available - 2004 |
Principles and Reactions of Protein Extraction, Purification, and ... Hafiz Ahmed No preview available - 2017 |
Common terms and phrases
absorbance acetic acid activity added addition Advantages affinity alkaline allow amine amino ammonium amount antibody antigen applications assay binding Biochem blot blue bound buffer carbohydrate cells Centrifuge charge chromatography color column common complex concentration conjugate containing coupling cross-linker described detection detergent determine diluted Dissolve electrophoresis elution enzyme equilibrating example extraction Figure final fixed fluorescent fractionation glucose glycoproteins gram groups immobilized immune Incubate interactions labeled lectins ligand matrix measured membrane method mg/ml mixture molecular weight molecules obtained performed peroxidase phase phosphate plate precipitate Prepare presence primary Procedure protein purification range reaction reagent reducing remove resulting room temperature salt sample sensitive separation silver sodium soluble solution specific staining standard step structure substrate surface Table techniques tissue transfer tube usually volume Wash Western blot