G ProteinsTechniques of Analysis
David R. Manning
CRC Press, May 19, 1999 - Medical - 320 pages
Incorporating a bench-top format, G Proteins: Techniques of Analysis covers essential methods - with a commitment to those techniques of proven and current utility.
G Proteins: Techniques of Analysis includes expression and functional analysis of G proteins; evaluation of covalent modifications and other regulatory phenomena; and, mapping pathways established among receptors, G proteins, and effectors.
Including contributors from key institutions, each author offers clear instructions to establish a synthesized, concise and consistent approach to each chapter, beneficial to students and professionals as well.
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Acad adenylyl cyclase ADP-ribosylation agonist aliquots analysis antibody antisense RNA bait binding Biol buffer cDNA cell lines centrifugation Chem cholera toxin coli concentration containing culture described detected detergent dilution domain EDTA effector elution encoding expression fatty acid Figure fractions function G protein-coupled receptors GAP activity GAP assays gene Gilman Go subunits Go-GTP GTPase GTPYS H]myristate H]palmitate heterotrimeric G proteins hydrolysis immunoblotting immunoprecipitation incubation inhibition injection interaction isoproterenol labeling lanes mammalian medium membrane protein method mg/ml microinjection molecular mutant myristoylated Natl Ni-NTA column nitrocellulose nucleotide ODNs palmitate palmitoylation panel pellet peptide pertussis toxin phospholipase plasmid prepared protein subunits protocol purified QT6 cells radiolabeled reagents receptor residues Resuspend sample SDS-PAGE sequence serum Sf9 cells signaling solution specific steady-state Sternweis stimulation Stock supernatant target techniques toxin substrates transducin transfected trypsin tube two-hybrid ug/ml vector volume wash Western blotting yeast