the injections were discontinued, was twenty-three hundred forty grams, a loss of four hundred ten grams, or nearly fifteen per cent. The daily urine of the fore period measured two hundred seventyfive cubic centimeters; that of the treatment period fluctuated within wide limits, but averaged two hundred forty-three cubic centimeters; that of the after period of eight days averaged one hundred seventy cubic centimeters, and after this the volume returned to normal. The total ash and nitrogen increased during the treatment, and remained above the normal for three days longer and then gradually fell to the normal. The animal apparently recovered fully, though we have no record of its subsequent history.3

Rabbit No. 6.--Chart III shows the temperature curve in this animal. It was given subcutaneously two hundred milligrams of the crude soluble typhoid poison every two hours, from 7 A. M. to 9 P. M., from May 3 to May 18, 1909.

Rabbit No. 7.-This animal received subcutaneous injections of egg-white diluted with an equal volume of five-tenths per cent phenol. The treatment continued from May 27 to June 15, 1909. The initial dose was two cubic centimeters of the dilution every two hours from 7 A. M. to 9 P. M. This was doubled June 1, and again June 4. The animal received forty doses of two cubic centimeters each, twenty doses of four cubic centimeters each, and eighty-two doses of eight cubic centimeters each; in all eight hundred sixteen cubic centimeters. Albumin appeared in the urine when the dose was increased to four cubic centimeters, and continued until the eleventh day after the injections were discontinued. It was shown by sensitizing guinea-pigs with the urine that it contained both eggwhite and serum albumin. Chart IV shows the record of this rabbit.

THE PRODUCTION OF FEVER BY INTRAABDOMINAL INJECTIONS OF PROTEINS. Large doses of egg-white, single or repeated, injected. into the abdominal cavity of a nonsensitized rabbit, cause no elevation of temperature; on the contrary, if a close and long-continued watch of the temperature be kept after such injections, it will often be found to be subnormal. In August, 1909, we injected the whites of three eggs, measuring eighty cubic centimeters, into the abdominal cavity of a rabbit. The temperature was taken every two hours from 8 A. M. to 6 P. M. from the date of the injection (August 22) to September 6. There was at no time any elevation of temperature above that of the fore period, and the morning temperature fell as low as 97°, and on one day to 96.6°. The animal lost one-fifth of its initial weight. The urine averaged normal in amount, and at no time did it contain albumin.

In contrast to this, five-tenths cubic centimeters of egg-white filtered through cotton and injected intraabdominally induced the temperature recorded in Chart V.

Chart II is omitted on account of being too large for insertion.

Cents fores produced in a rather by the subcutancom injection of 200 mg doses typhoid poison.

THE PRODUCTION OF FEVER BY INTRAVENOUS INJECTIONS OF PROTEINS. The intravenous injection of a large amount of egg-white in a single or in repeated doses in nonsensitized rabbits does not cause any marked elevation of temperature. Having kept a rabbit under observation for three days and finding that its temperature at no time reached 102°, we injected four cubic centimeters of a dilution of egg-white with an equal volume of physiological salt solution into its ear vein every two hours, from 8 A. M. to 6 P. M. This dilution had been passed through a Berkfeld filter and each cubic centimeter contained twenty-six milligrams of protein, as ascertained by a nitrogen determination. This dosage was continued for six days. During the greater part of this time the temperature, which was taken before each injection, remained normal, sometimes subnormal, and only once did it reach 102°. The dose was then increased to ten cubic centimeters, and continued at this for four days. Twenty-four hours after the increase there was irregular, but not marked, elevation of temperature, the highest point reached being 104.4. The animal remained apparently well; its greatest weight was observed at the time when the largest injections were being given, and at the same time the volume of urine greatly increasedfrom one hundred fourteen cubic centimeters, the average of the fore period, to as much as six hundred fifty cubic centimeters. Albumin was not found in the urine at any time. This experiment was continued from August 6 to August 19, 1909. In March, 1910, a single injection of five cubic centimeters of a like dilution of eggwhite was followed within a few hours by a gradual rise in temperature to 105.8°.

Fever is induced in fresh rabbits by repeated injections of small doses of egg-white, and the most striking results are obtained when the size of the dose is gradually and uniformly increased.

Chart VI shows the results when the initial dose was twenty-six milligrams of protein, and this was increased by the same amount hourly. This animal died with a slight convulsive movement. The urine of the day and that found in the bladder after death was tested for albumin, with negative results.

With an initial dose of thirteen milligrams, increased in like manner, the temperature went as high as in the preceding, but the animal recovered. With an initial dose of six and five-tenths milligrams of protein the highest temperature reached was 104.6°. After an interval of one hundred seventy-four days this animal was treated with a like dilution, with a fatal ending.

With an initial dose of three and twenty-five hundredths milligrams of protein, the highest temperature reached was 104°. After an interval of one hundred forty days this animal was treated with a like dilution. After the second injection of the second series Cheyne-Stokes respiration set in, and continued through the day,

but the injections were repeated hourly until 6 P. M., and the animal recovered.

When but one dose a day is given and the size of the dose increased each day, an intermittent or a remittent fever, according to the size of the dose, may be induced. In one case of this kind the dose was given at 10 each morning. The initial dose was three cubic centimeters each day. A well-marked intermittent fever of mild type was established. The temperature rose from two to four hours after each injection, returned to normal in the afternoon, and so remained until the next injection. As the doses grew larger the tendency was to take the remittent type, so that at no time of the day did the fall reach the normal.

We have found that the exact size of the dose necessary to induce marked fever in rabbits varies decidedly in different batches, and somewhat in individuals. The dilution containing twenty-six milligrams of protein per cubic centimeter may kill without causing any elevation of temperature. In these instances, higher dilutions are necessary. In one consignment of rabbits we had to reduce the dilution to six and five-tenths of protein per cubic centimeter before we secured a satisfactory fever curve. Stronger solutions lowered the temperature and killed. These differences in response to injections of egg-white seem to be characteristic of certain groups or batches of rabbits. We buy our animals in lots of twenty-five or more, and in diverse localities. If one out of a given lot responds to a certain dose, others of the same lot are likely to behave in a similar manner. Whether this is due to differences in food or breed

we have not determined. Possibly age is an individual factor.

The anatomical findings after acute poisoning with egg-white have been studied for us by Morse, from whose report we make the following brief abstract: The peritoneal cavity contains a small amount of blood-stained fluid. The pleural and pericardial cavities contain no excess of fluid. The fat of the anterior mediastinum shows a few petechial hemorrhages. The thymus is large and edematous, spreading over the anterior mediastinum, covering the great vessels and presenting hundreds of miliary hemorrhages. The heart is moderately dilated and filled with red clots. The valves are normal. The myocardium is dark and drips blood too freely. The lungs are slightly darker than normal. There are no consolidated areas. The kidneys are congested and drop blood. on section. The adrenals are apparently normal, as are the stomach, intestines, and bladder. The liver is congested and bleeds freely on section. In the retroperitoneum there is a suffusion of blood through the cellular tissue, partly involving the head of the pancreas. The brain appears normal, but section. shows some congestion.

The most striking microscopic findings are as follows: In the muscles of the heart. there are numerous miliary hemorrhages,