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THE object of this small work is to lay before the practitioner and student of medicine, a few concise and simple methods, by which the various tissues of the body may be prepared for examination with the microscope.

I do not claim any originality in these methods, but I recommend them from my own personal experience as the best, easiest, and cheapest to carry out.

The use of dyes for staining the tissues is a comparatively new branch of the science of Histology, and its value is being proved every day.

I have been engaged in experiments with all the various colouring agents for a long time, and in trying their effect in double and treble staining, and I have given the result of those which have proved most successful hitherto. I have also added a list of the aniline dyes, those soluble in water, and those soluble in spirit, which will be found very useful by anyone wishing to make experiments in staining.

Briefly, but sincerely, I offer my best thanks to Dr. Klein, F.R.S., for the assistance which he has at all times freely and generously given me.

H. G.

94, GOWER STREET,

October 1, 1880.

PREFACE TO THE THIRD EDITION.

THIS edition has been thoroughly revised and a quantity of new matter introduced.

The various staining and other methods given will be found those most useful for the work of the present day.

44, CHARLEVILLE Road,

WEST KENSINGTON,

August 1, 1885.

H. G.

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